Photon-counting H33D detector for biological fluorescence imaging

Photon-Counting H33D Detector for Biological Fluorescence Imaging.

We have developed a photon-counting High-temporal and High-spatial resolution, High-throughput 3-Dimensional detector (H33D) for biological imaging of fluorescent samples. The design is based on a 25 mm diameter S20 photocathode followed by a 3-microchannel plate stack, and a cross delay line anode. We describe the bench performance of the H33D detector, as well as preliminary imaging results o...

Photon -counting Reticontm Detector

For applications in faint -object spectroscopy at the Mt. Hopkins Observatory, a photon counting detector has been built using a dual 936 Reticon diode array fibre -optically coupled to a high -gain image intensifier package. The electronic signal processing follows closely the design of Shectman; the diode array is scanned every millisecond, each frame is subtracted from the previous to minimi...

Phasor-based single-molecule fluorescence lifetime imaging using a wide-field photon-counting detector.

Fluorescence lifetime imaging (FLIM) is a powerful approach to studying the immediate environment of molecules. For example, it is used in biology to study changes in the chemical environment, or to study binding processes, aggregation, and conformational changes by measuring Förster resonance energy transfer (FRET) between donor and acceptor fluorophores. FLIM can be acquired by time-domain me...

Fluorescence lifetime imaging by time-correlated single-photon counting.

We present a time-correlated single photon counting (TCPSC) technique that allows time-resolved multi-wavelength imaging in conjunction with a laser scanning microscope and a pulsed excitation source. The technique is based on a four-dimensional histogramming process that records the photon density over the time of the fluorescence decay, the x-y coordinates of the scanning area, and the wavele...

Single Photon Counting X-Ray Micro-Imaging of Biological Samples